This Scheme enables the assessment of 4 archival positive fluorescence images. The specimen types and fluorescent markers required for submission are not specified and Participants are permitted to submit any representative image showing positive staining. The magnification of each image should be appropriate to its purpose, the purpose being an overview of the biopsy as a whole or demonstration of one specific diagnostic feature.

Staining Criteria Handbook available in Members' Area

Rationale

DIF is carried out in many histopathology and some immunology centres in the UK and overseas. Images that are taken from tissue sections prepared for DIF should highlight specific features in the tissue that inform the diagnostic process and together give a full and clear representation of the fluorescence findings contained within the tissue. The images must be in focus and of sufficient contrast to clearly demonstrate the features of immunofluorescence examination.

Submission

4 positive fluorescence images after the date indicated on the delivery letter, irrespective of number of cases, provided that at least 4 separate positive conjugate examples from a Participants in-house repertoire are represented.

Reports

• Individualised

• Generic Best Method

• Generic Image